Active and Inactive Regions of Nuclear Chromatin as Revealed by Electron Microscope Autoradiography.
نویسندگان
چکیده
* The work described in this paper was sponsored by the U.S. Atomic Energy Commission. t On leave of absence from the Department of Biochemistry, University College, London. 1 Rabin, B. R., and P. W. Trown, these PROCEEDINGS, 51, 497 (1964). 2 Ellman, G. L., Arch. Biochem. Biophys., 82, 70 (1959). 3Rabin, B. R., D. F. Shaw, N. G. Pon, J. M. Anderson, and M. Calvin, J. Am. Chem. Soc., 80, 2528 (1958). 4Yankeelov, J. A., Anal. Biochem., 6, 287 (1963). 5 Hospelhorn, V. D., Anal. Biochem., 2, 180 (1961). 6 Rohr, W., and A. L. Bassham, in preparation. 7Hirs, C. H. W., J. Biol. Chem., 219, 611 (1956). 8 Crowley, G. T., V. Moses, and J. Ulirich, J. Chromatog., 12, 219 (1963). 9 Trown, P. W., in preparation.
منابع مشابه
Nuclear morphology and the ultrastructural localization of deoxyribonucleic acid synthesis during interphase.
The ultrastructural localization of deoxyribonucleic acid (DNA) synthesis was studied by electron-microscope autoradiography in human transforming lymphocytes, embryonic lung fibroblasts, epithelial cells and normoblasts. Euchromatin was found to be active in DNA synthesis in all cell types studied, whereas heterochromatin was inactive. However, DNA synthesis was also prominent in the regions w...
متن کاملSmall nuclear RNA localization during mitosis. An electron microscope study
The localization of small nuclear ribonucleic acids (snRNAs) during mitosis in Amoeba proteus was studied by high voltage (1,000 kV) electron microscope autoradiography. By suitable micromanipulations, the snRNA's, labeled with [3H]uridine, were made to be the only radioactive molecules in the cell and thus easy to follow autoradiographically. During interphase the snRNA label, which is almost ...
متن کاملUptake in Pseudomonas Tabaci
Scintillation studies on the uptake of Ni by Pseudomonas tabaci demonstrate an incorporation of approximately 2-5 nmol per 10'° bacterial cells, in medium containing 12nmol (8-3 /iCi) per ml. Over 80% of the incorporated Ni is lost from the cells during washing, fixation and dehydration with ethanol. The remaining insoluble (bound) Ni has the highest level in cells fixed in acetic acid/ethanol ...
متن کاملIn situ hybridization at the electron microscope level: hybrid detection by autoradiography and colloidal gold
In situ hybridization has become a standard method for localizing DNA or RNA sequences in cytological preparations. We developed two methods to extend this technique to the transmission electron microscope level using mouse satellite DNA hybridization to whole mount metaphase chromosomes as the test system. The first method devised is a direct extension of standard light microscope level using ...
متن کاملModulation by exogenous histones of phosphorylation of non-histone nuclear proteins in isolated rat liver nuclei.
The phosphorylation of specific non-histone proteins in isolated rat liver nuclei is modified by the addition of exogenous histone fractions. The added histones are quantitatively adsorbed and their presence in the chromatin has been verified by electron microscope autoradiography. Histone fractions Fl and FZAl stimulate phosphorylation of different nuclear phosphoproteins. Fl stimulates the ph...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 52 شماره
صفحات -
تاریخ انتشار 1964